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SciLifeLab Cryo-EM Course on Tomography - 1 ECTS

modv0000Welcome to an advanced course in CryoEM Tomography!

Organiser and collaborators:
The Cryo-EM Swedish National Facility - Umeå node

In collaboration with
- SciLifeLab
- CryoNet - the Nordic Network in cryo-EM
- The Laboratory for Molecular Infection Medicine Sweden (MIMS)
- Umeå Centre for Microbial Research (UCMR)

Date: 26-28th March 2019, 3 days

Venue:
Umeå University, Umeå Core Facility of Electron Microscopy (UCEM), Electronmicroscopy Building and Chemical Biological Centre (KBC)

Important dates:
-
Application open: 2018-12-01 (please visit the page again - for application information)
- Application deadline: 2019-02-24

Responsible teacher/s:
-
Linda Sandblad, head of Cryo-EM Facility at Umeå University
- Marta Carroni, head of Cryo-EM Facility at Stockholm University
- Johanna Höög, associate professor, University of Gothenburg

Invited lecturer and course instructors:
-
Cindi Lynn Schwartz,  National Institute of Allergy and Infectious Diseases, NIAID Rocky Mountains Laboratory, Hamilton, Montana, USA
- Giulia Zanetti, Birkbeck University of London, UK

Contact information for questions regarding the course:
Linda Sandblad, head of the Cryo-EM Facility, Umeå Core Facility for Electron Microscopy (UCEM), Umeå University, This email address is being protected from spambots. You need JavaScript enabled to view it.

Course fee:
1200 SEK. The fee is not paid during registration. After application acceptance and invitation to attend, all participants will be asked to provide us with address and ref number for a course fee invoice.

Lunches and coffee are included in the course fee.

Course content:
SciLifeLab Cryo-EM facility and CryoNET arrange a course on tomography in Umeå, organized by Umeå Core facility for Electron Microscopy, associated to the KBC, MIMS and UCMR.

During the course we will introduce sample preparation and cryo-EM data acquisition for tomography. Practical focus will be on 3D image reconstruction and analysis using the IMOD software package. Also, the concepts of cryo-EM subtomogram averaging will be demonstrated. All participant will work with IMOD on individual work stations in a computer lab to practice electron tomography image alignment, 3D reconstruction, refinement and averaging methods, modeling of protein complexes and cellular volumes and graphical presentation of 3D image data. The workshop and methods are aimed for structure, cell biology and research in related fields.

Entry requirements
This course aims for advanced training for researchers with earlier experiences in electron microscopy and/or 3D image analyzes. Participants will be selected depending on the motivation stated in the application form.

Related Links:
The National Cryo-EM Facility

The Umeå Core Facility for Electron Microscopy (UCEM)

Emmanuelle Charpentier:
MIMS is characterized by
ECharpentier about MIMS 2015 1

Emmanuelle Charpentier, Alumna at MIMS, now Director of the Max Planck Unit for the Science of Pathogens and Director of the Department of Regulation in Infection Biology, Max Planck Institute for Infection Biology, Berlin, Germany.

Link to the Emmanuelle Charpentier Lab

footer all slides 2014-02-06


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