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Atomic Force Microscopy (AFM) facility at the Department of Molecular Biology

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This facility counts with a Bruker multimode AFM with the fully automatized ScanAsyst system, which requires minimal supervision and zero parameter setting during operations. The system is currently calibrated to observe objects in the micrometer range with subnanometric resolution. The small vertical range of 20 μm is purposely designed for small cells and for molecular complexes including single proteins and DNA.

Compared to other imaging techniques it has the advantage that it does not requires fixation and sample preparation is minimal. Imaging in liquid under native conditions is also possible.

Team leader and Facility manager:
David A. Cisneros, Dr. rer nat.

Read more about the research in David Cisneros' group

Contact e-mail:
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Gallery AFM



 

Biochemical Imaging Centre at KBC

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The national centre for advanced fluorescence imaging, called The Biochemical Imaging Centre Umeå (BICU), was founded with support from the Swedish Research Council in order to establish a node of excellence for fluorescence super-resolution and live cell imaging. The centre includes equipment for combined use of different advanced imaging techniques to visualise the same sample in multiple ways.

Nikon A1R webThe instrumentation includes a spinning disk microscope, for extremely high-speed live cell imaging and a confocal microscope with resonant scanner. Both microscopes are fully equipped for photo-bleaching (FRAP) and photo-quenching (FRET) experiments and TIRF (total internal reflection fluorescence) microscopy. The equipment is completed with a STORM-unit that allows super-resolution imaging of structures and micromanipulators down to 20nm.

The facility is also equipped with an Atomic Force Microscope (AFM) that provides high resolution and force measurements to test specific interactions between the biological molecules in their natural environment and enables to get insight into the geometry, elasticity and dynamic behavior at the level of single molecules or single cells.

Complementary to the imaging instruments, the centre provides equipment for precise affinity measurements on surfaces or in solution using Surface Plasmon Resonance, Ligand-tracer and Isothermal Titration Calorimetry.

BICU closely collaborates with the Umeå Core Facility for Electron Microscopy, UCEM, to provide correlative imaging technology (techniques, methods and know-how) and an optimal workflow for CLEM (Correlative Light and Electron Microscopy). Both infrastructures are driving forces in the Swedish National Microscopy Infrastructure (NMI).

The organization:

Director:
Richard Lundmark, assoc. prof This email address is being protected from spambots. You need JavaScript enabled to view it.

Facility Manager:
Irene Martinez, PhD This email address is being protected from spambots. You need JavaScript enabled to view it.

Host department:
Medical Biochemistry and Biophysics, KBC, Umea Universitet

Detailed information on the Webpage of the Biochemical Imaging Centre Umeå, BiCU



 

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