Microscopy Facilities related to MIMS
The national centre for advanced fluorescence imaging, called The Biochemical Imaging Centre Umeå (BICU), was founded with support from the Swedish Research Council in order to establish a node of excellence for fluorescence super-resolution and live cell imaging. The centre includes equipment for combined use of different advanced imaging techniques to visualise the same sample in multiple ways.
The instrumentation includes a spinning disk microscope, for extremely high-speed live cell imaging and a confocal microscope with resonant scanner. Both microscopes are fully equipped for photo-bleaching (FRAP) and photo-quenching (FRET) experiments and TIRF (total internal reflection fluorescence) microscopy. The equipment is completed with a STORM-unit that allows super-resolution imaging of structures and micromanipulators down to 20nm.
The facility is also equipped with an Atomic Force Microscope (AFM) that provides high resolution and force measurements to test specific interactions between the biological molecules in their natural environment and enables to get insight into the geometry, elasticity and dynamic behavior at the level of single molecules or single cells.
Complementary to the imaging instruments, the centre provides equipment for precise affinity measurements on surfaces or in solution using Surface Plasmon Resonance, Ligand-tracer and Isothermal Titration Calorimetry.
BICU closely collaborates with the Umeå Core Facility for Electron Microscopy, UCEM, to provide correlative imaging technology (techniques, methods and know-how) and an optimal workflow for CLEM (Correlative Light and Electron Microscopy). Both infrastructures are driving forces in the Swedish National Microscopy Infrastructure (NMI).
Medical Biochemistry and Biophysics, KBC, Umea Universitet
Microscopy at UCMR/MIMS and the Department of Molecular Biology
Policy and pricelist
The goal is to provide researchers at Umeå University with advanced imaging technology in combination with technical support, and imaging-related expert advice for research projects.
The emphasis is put on state-of-the-art technology, including live cell imaging, confocal microscopy, atomic force microscopy (AFM) and IVIS, Live Animal Imaging System for experimental animal models. The facility can also offer instrumentation for more conventional techniques, such as brigthfield and fluorescence microscopy.
Currently, most of the microscopes can be booked online from the Department of Molecular Biology, but you need to have a valid research group account.
Go to: http://scorpio.molbiol.umu.se/Artologik
The booking system is available for external users from any of the Nikon 90i microscope computers at the department.
External users will be charged by hour.