Postdoc opportunities in cryo-electron tomography and in vitro reconstitution of human RNA virus replication
Carlson lab, Department of Medical Biochemistry and Biophysics
Wallenberg Centre for Molecular Medicine, Umeå University, Sweden and part of the Umeå Centre for Microbial Research, UCMR.
Two postdoctoral fellowships are available immediately in the lab of Lars-Anders Carlson at Umeå University, Sweden. The lab studies the genome replication of human RNA viruses using cryo-electron tomography, in vitro membrane reconstitution and fluorescence microscopy.
1. Structural studies of viral replication complexes in cells. The postdoctoral fellow will use cryo-FIB-milling and cryo-electron tomography to structurally characterise replication complexes inside virus-producing cells. The fellow will have full access to Umeå's new state-of-the-art cryo-EM facility (including a Scios FIB/SEM instrument and a Titan Krios with phase plate, energy filter and direct detectors), as well as excellent resources for cell biology and virology. A collaboration with an internationally leading virology lab is established.
Reference: FS 2.1.6-305-17
2. Synthetic biology of membrane-localised viral genome replication. The postdoctoral fellow will reconstitute viral replication complexes from individual purified proteins, RNA and synthetic giant unilamellar vesicles (GUVs). The ensuing dynamics will be studied by quantitative fluorescence microscopy at Umeå's high-end fluorescence microscopy facility BiCU. Structural studies of purified complexes may also be conducted using cryo-EM. A collaboration with a leading virology lab is established and excellent resources for biochemistry are available within the department.
Reference: FS 2.1.6-306-17
The recruited postdocs will benefit from Umeå's strength in infection biology including the Nordic EMBL node MIMS, and from Umeå's friendly, non-hierarchical atmosphere. The 2014 European capital of culture, Umeå is the largest city in northern Sweden yet small enough to be bicycle and pedestrian friendly. It is known both for its alternative culture scene(s) and its proximity to the Scandinavian wilderness.
The successful applicants must hold a PhD at the time of recruitment, and should have a solid training in a field such as biophysics, cryo-electron microscopy, high-end fluorescence microscopy, structural biology or biochemistry. While previous experience in the specific methods of the projects is beneficial, your ambitiousness and enthusiasm will be more important and we thus also welcome applicants from different backgrounds.
The duration of the fellowships is two years. They are available immediately and will remain open until filled, with an intial deadline of 2017-03-31. Your application should contain a statement of interest, CV including publication list and contact information to 3 references, one of whom should be your PhD advisor.
We look forward to receiving your application!
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