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Vasili Hauryliuk on Google Scholar


Roghanian M, Van Nerom K, Takada H, Caballero-Montes J, Tamman H, Dzhygyr I, Talavera A, Kudrin P, Atkinson GC, Ekström S, Garcia-Pino A, Hauryliuk V
(p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains
Molecular Cell, 2021 81(16), 3310-3322.e6

Mojr V, Roghanian M, Tamman H, Pham DDD, Petrová M, Pohl R, Takada H, Van Nerom K, Ainelo H, Caballero-Montes J, Jimmy S, Garcia-Pino A, Hauryliuk V, Rejman D
Nonhydrolysable Analogues of (p)ppGpp and (p)ppApp Alarmone Nucleotides as Novel Molecular Toolsd
ACS Chemical Biology, 2021

Takada H, Crowe-McAuliffe C, Polte C, Sidorova ZY, Murina V, Atkinson GC, Konevega AL, Ignatova Z, Wilson DN, Hauryliuk V

RqcH and RqcP catalyze processive poly-alanine synthesis in a reconstituted ribosome-associated quality control system
Nucleic Acids Res. 2021 doi: 10.1093/nar/gkab589

Jurėnas D, Payelleville A, Roghanian M, Turnbull KJ, Givaudan A, Brillard J, Hauryliuk V, Cascales E

Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA
Nucleic Acids Res. 2021 doi: 10.1093/nar/gkab608

Kurata T, Brodiazhenko T, Alves Oliveira SR, Roghanian M, Sakaguchi Y, Turnbull KJ, Bulvas O, Takada H, Tamman H, Ainelo A, Pohl R, Rejman D, Tenson T, Suzuki T, Garcia-Pino A, Atkinson GC, Hauryliuk V

RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis
Mol Cell. 2021 Jun 23:S1097-2765(21)00452-4

Crowe-McAuliffe C, Murina V, Turnbull KJ, Kasari M, Mohamad M, Polte C, Takada H, Vaitkevicius K, Johansson J, Ignatova Z, Atkinson GC, O'Neill AJ, Hauryliuk V, Wilson DN

Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogenss
Nature Communications, 2021 12 3577

Takada H, Roghanian M, Caballero-Montes J, Van Nerom K, Jimmy S, Kudrin P, Trebini F, Murayama R, Akanuma G, Garcia-Pino A, Hauryliuk V

Ribosome association primes the stringent factor Rel for tRNA-dependent locking in the A-site and activation of (p)ppGpp synthesis
Nucleic Acids Research, 2021 49(1) 444-457

Crowe-McAuliffe C, Takada H, Murina V, Polte C, Kasvandik S, Tenson T, Ignatova Z, Atkinson G, Wilson DN, Hauryliuk V
Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP
Molecular Cell, 2021 81(1) 115-126


Kaldalu N, Hauryliuk V, Turnbull K, La Mensa A, Putrinš M, Tenson T
In Vitro Studies of Persister Cells
Microbiol Mol Biol Rev 2020 84(4):e00070-20

Irie Y, La Mensa A, Murina V, Hauryliuk V, Tenson T, Shingler V
Hfq-Assisted RsmA Regulation Is Central to Pseudomonas aeruginosa Biofilm Polysaccharide PEL Expression
Front. Microbiol., 17 November 2020

Wilson DN, Hauryliuk V, Atkinson GC, O'Neill AJ
Target protection as a key antibiotic resistance mechanism
Nat Rev Microbiol, 2020 doi: 10.1038/s41579-020-0386-z.

Jimmy S, Saha CK, Kurata T, Stavropoulos C, Oliveira SRA, Koh A, Cepauskas A, Takada H, Rejman D, Tenson T, Strahl H, Garcia-Pino A, Hauryliuk V, Atkinson GC
A Widespread Toxin-Antitoxin System Exploiting Growth Control via Alarmone Signaling
Proc Natl Acad Sci U S A, 2020 117(19): 10500–10510

Tamman H, Van Nerom K, Takada H, Vandenberk N, Scholl D, Polikanov Y, Hofkens J, Talavera A, Hauryliuk V, Hendrix J, Garcia-Pino A
A Nucleotide-Switch Mechanism Mediates Opposing Catalytic Activities of Rel Enzymes
Nat Chem Biol, 2020 doi: 10.1038/s41589-020-0520-2

Takada H, Roghanian M, Murina V, Dzhygyr I, Murayama R, Akanuma G, Atkinson GC, Garcia-Pino A, Hauryliuk V
The C-Terminal RRM/ACT Domain Is Crucial for Fine-Tuning the Activation of 'Long' RelA-SpoT Homolog Enzymes by Ribosomal Complexes
Front Microbiol. 2020, 11:277


Van Nerom K, Tamman H, Takada H, Hauryliuk V, Garcia-Pino A
Crystallization and X-ray analysis of the Rel stringent factor from Thermus thermophilus
Acta Cryst. 2019 F75, 561-569

Kasari V, Pochopien AA, Margus T, Murina V, Turnbull K, Zhou Y, Nissan T, Graf M, Nováček J, Atkinson GC, Johansson MJO, Wilson DN, Hauryliuk V
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
Nucleic Acids Research, 2019 doi: 10.1093/nar/gkz600

Turnbull KJ, Dzhygyr I, Lindemose S, Hauryliuk V, Roghanian M
Intramolecular interactions dominate the autoregulation of Escherichia coli stringent factor RelA
Frontiers in Microbiology 2019 10:1966

Zbornikova E, Knejzlik Z, Hauryliuk V, Krasny L, Rejman D
Analysis of Nucleotide Pools in Bacteria Using HPLC-MS in HILIC Mode
Talanta 2019

Kasari V, Margus T, Atkinson GC, Johansson MJO, Hauryliuk V
Ribosome profiling analysis of eEF3-depleted Saccharomyces cerevisiae
Sci Rep. 2019 Feb 28;9(1):3037


Brodiazhenko T, Johansson MJO, Takada H, Nissan T, Hauryliuk V, Murina V
Elimination of Ribosome Inactivating Factors Improves the Efficiency of Bacillus subtilis and Saccharomyces cerevisiae Cell-Free Translation Systems
Front Microbiol. 2018 Dec 18;9:3041

Goormaghtigh F, Fraikin N, Putrinš M, Hallaert T, Hauryliuk V, Garcia-Pino A, Sjödin A, Kasvandik S, Udekwu K, Tenson T, Niilo Kaldalu N, Van Melderen L
Reply to Holden and Errington, “Type II Toxin-Antitoxin Systems and Persister Cells”
mBio 2018, doi: 10.1128/mBio.018

Crowe-McAuliffe C, Graf M, Huter P, Takada H, Abdelshahid M, Nováček J, Murina M, Atkinson GC, Hauryliuk V, Wilson DN
Structural basis for antibiotic resistance mediated by the Bacillus subtilis ABCF ATPase VmlR
Proc Natl Acad Sci U S A 2018 pii: 201808535. doi: 10.1073/pnas.1808535115

Goormaghtigh F, Fraikin N, Putrinš M, Hallaert T, Hauryliuk V, Garcia-Pino A, Sjödin A, Kasvandik S, Udekwu K, Tenson T, Niilo Kaldalu N, Van Melderen L
Reassessing the role of type II toxin-antitoxin systems in formation of Escherichia coli type II persister cells
mBio 2018, 9: e00640-18

Kudrin P, Dzhygyr I, Ishiguro K, Beljantseva J, Maksimova E, Oliveira SRA, Varik V, Payoe P, Konevega AL, Tenson T, Suzuki T, Hauryliuk V
The ribosomal A-site finger is crucial for binding and activation of the stringent factor RelA
Nucleic Acids Research 2018, doi: 10.1093/nar/gky023

Manav MC, Beljantseva J, Bojer MS, Tenson T, Ingmer H, Hauryliuk V, Brodersen DE
Structural Basis for (p)ppGpp Synthesis by the Staphylococcus aureus Small Alarmone Synthetase RelP
Journal of Biological Chemistry 2018, doi: 10.1074/jbc.RA117.001374

Murina V, Kasari M, Hauryliuk V, Atkinson GC
Antibiotic resistance ABCF proteins reset the peptidyl transferase center of the ribosome to counter translational arrest
Nucleic Acids Research 2018, doi: 10.1093/nar/gky050


Hauryliuk V & Atkinson GC
Small Alarmone Synthetases as novel bacterial RNA-binding proteins
RNA Biology 2017, doi: 10.1080/15476286.2017.1367889

Varik V, Oliveira SR, Hauryliuk V, Tenson T
HPLC-based quantification of bacterial housekeeping nucleotides and alarmone messengers ppGpp and pppGpp
Scientific Reports 2017, 7:11022 

Beljantseva J, Kudrin P, Andresen L, Shingler V, Atkinson GC, Tenson T, Hauryliuk V
Negative allosteric regulation of Enterococcus faecalis small alarmone synthetase RelQ by single-stranded RNA
Proc Natl Acad Sci U S A. 2017, doi: 10.1073/pnas.1617868114

Beljantseva J, Kudrin P, Jimmy S, Ehn M, Pohl R, Varik V, Tozawa Y, Shingler V, Tenson T, Rejman D, Hauryliuk V
Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor
Scientific Reports 2017, 7:41839

Kudrin P, Varik V, Oliveira SR, Beljantseva J, Del Peso Santos T, Dzhygyr I, Rejman D, Cava F, Tenson T, Hauryliuk V
Subinhibitory Concentrations of Bacteriostatic Antibiotics Induce relA-Dependent and relA-Independent Tolerance to β-Lactams
Antimicrobial Agents Chemotherapy 2017, 61(4) doi: 10.1128/AAC.02173-16


Allas UL, Toom L, Selyutina A, Mäeorg U, Medina R, Merits A, Rinken A, Hauryliuk V, Kaldalu N, Tenson T
Antibacterial activity of the nitrovinylfuran G1 (Furvina) and its conversion products
Scientific Reports 2016, 6:36844

Andresen L, Tenson T, Hauryliuk V
Cationic bactericidal peptide 1018 does not specifically target the stringent response alarmone (p)ppGpp
Scientific Reports 2016, 6:36549

Andresen L, Varik V, Tozawa Y, Jimmy S, Lindberg S, Tenson T, Hauryliuk V
Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors
Scientific Reports 2016, 6:35824

Monteferrante CG, Jirgensons A, Varik V, Hauryliuk V, Goessens WH, Hays JP
Evaluation of the characteristics of leucyl-tRNA synthetase (LeuRS) inhibitor AN3365 in combination with different antibiotic classes
Eur J Clin Microbiol Infect Diseases 2016, 35(11):1857-1864

Kaldalu N, Hauryliuk V, Tenson T
Persisters - as elusive as ever
Applied Microbiology and Biotechnology 2016, DOI: 10.1007/s00253-016-7648-8

Arenz S, Abdelshahid M, Sohmen D, Payoe R, Starosta AL, Berninghausen O,  Hauryliuk V, Beckmann R, Wilson DN
The stringent factor RelA adopts an open conformation on the ribosome to stimulate ppGpp synthesis
Nucleic Acids Res. 2016, doi: 10.1093/nar/gkw470

Varik V, Oliveira SRA, Hauryliuk V, Tenson T
Composition of the outgrowth medium modulates wake-up kinetics and ampicillin sensitivity of stringent and relaxed Escherichia coli
Scientific Reports 2016, 6:22308

Kuzmenko A, Derbikova K, Salvatori R, Tankov S, Atkinson GC, Tenson T, Ott M,  Kamenski P, Hauryliuk V
Aim-less translation: loss of Saccharomyces cerevisiae mitochondrial translation initiation factor mIF3/Aim23 leads to unbalanced protein synthesis
Scientific Reports. 2016, 6: 18749


Hauryliuk V, Atkinson GC, Murakami KS, Tenson T, Gerdes K
Recent functional insights into the role of (p)ppGpp in bacterial physiology
Nat Rev Microbiol. 2015 13(5):298-309

Borg A, Holm M, Shiroyama I, Hauryliuk V, Pavlov M, Sanyal S, Ehrenberg M
Fusidic acid targets elongation factor G in several stages of translocation on the bacterial ribosome
Journal of Biological Chemistry 2015, 290(6):3440-54


Atkinson GC, Kuzmenko A, Chicherin I, Soosaar A, Tenson T, Carr M, Kamenski P, Hauryliuk V
An evolutionary ratchet leading to loss of elongation factors in eukaryotes
BMC Evolutionary Biology 2014; 14:35


Kuzmenko A, Atkinson GC, Levitskii S, Zenkin N, Tenson T, Hauryliuk V, Kamenski P 
Mitochondrial translation initiation: conservation and diversification
Biochimie 2013; 100, 132–140

Kuzmenko A, Levitskii SA, Vinogradova E, Atkinson GC, Hauryliuk V, Zenkin N, Kamenski P 
Protein biosynthesis in mitochondria
Biochemistry (Moscow) 2013; 78(8), 855-866

Li W, Atkinson GC, Thakor NS, Allas Ü, Lu CC, Chan KY, Tenson T, Schulten K, Wilson KS, Hauryliuk V, Frank J
Mechanism of tetracycline resistance by ribosomal protection protein Tet(O)
Nature Communications 2013; 4:1477 DOI: 10:1038/ncomms2470


Mitkevich AA, Shyp V, Petrushanko IYu, Soosaar A, Atkinson GC, Tenson T, Makarov AA, Hauryliuk V,
GTPases IF2 and EF-G bind GDP and the SRL RNA in a mutually exclusive manner
Scientific Reports 2012; 2:843 DOI:10.1038/srep00843

Shyp V, Tankov S, Ermakov A, Kudrin P, English BP, Ehrenberg M, Tenson T, Elf J, Hauryliuk V,
Positive allosteric feedback regulation of the stringent response enzyme RelA by its product
EMBO Reports 2012; 13, 835-839
discussed in Research highlights, Nat. Chem. Bio. 2012; 8(9): 738-738

Atkinson GС, Kuzmenko A, Kamenski P, Vysokikh MY, Lakunina V, Tankov S, Smirnova E, Sooraar A, Tenson T, Hauryliuk V
Evolutionary and genetic analyses of mitochondrial translation initiation factors identify the missing mitochondrial IF3 in S. cerevisiae
Nucleic Acids Res. 2012; 40(13), 6122-6134


Kuzmenko A, Tankov S, English B, Tarassov I, Tenson T, Kamenski P, Elf J, Hauryliuk V,
Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40
Scientific Reports 2011; 1:195 DOI:10.1038/srep00195

Atkinson GC, Tenson T, Hauryliuk V,
The RelA/SpoT Homolog (RSH) superfamily: distribution and functional evolution of ppGpp synthetases and hydrolases across the tree of life
PLoS ONE 2011; 6(8): e23479

English B, Hauryliuk V, Sanamard A, Tankov S, Dekker N, Elf, J
Single molecule Investigations of the stringent response machinery in living bacterial cells
Proc Natl Acad Sci U S A 2011; 108(31): E359-364

F1000-ranked article, discussed in PNAS Author Summary, in In vitro and in vivo singe-molecule fluorescence imaging of ribosome-catalyzed protein synthesis, Curr. Opinion in Chem. Biol. 2011; doi:10.1016, in Signalling in space and time: systems dynamics of intracellular communication, EMBO Reports 2011; 12(9): 877-879 and in Research highlights, Biopolymers 2011 Oct; 95(10):iii-iv

Atkinson GC, Hauryliuk V, Tenson T
An ancient family of SelB elongation factor-like proteins with a broad but disjunct distribution across archaea
BMC Evol Biol. 2011; 11:22


Chen L, Muhlard D, Hauryliuk V, Zhihong C, Lim KM, Shyp V, Parker R, Song H
Dom34-Hbs1 complex and implications for its role in No-Go decay
Nat Struct & Mol Biol. 2010 Oct; 17(10):1233-40

Mitkevich VA, Ermakov A, Kulikova AA, Tankov S, Shyp V, Soosar A, Tenson T,  Ehrenberg M, Hauryliuk V
Thermodynamic charachterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP and ppGpp
J Mol Biol. 2010 Sep; 402:838-846

English B, Sanamrad A, Tankov S, Hauryliuk V, Elf J
Tracking of individual freely diffusing fluorescent protein molecules in the bacterial cytoplasm
arXiv q-bio: 1003.2110, 2010

Kononenko AV, Mitkevich VA, Atkinson GC, Tenson T, Dubovaya VI, Frolova LY, Makarov AA, Hauryliuk V.
GTP-dependent structural rearrangement of the eRF1:eRF3 complex and eRF3 sequence motifs essential for PABP binding.
Nucleic Acids Res. 2010 Feb; 38(2):548-58

discussed in Applications of isothermal titration calorimetry in pure and applied research - survey of literature from 2010, J Mol Recognition. 2012 25(1): 32-52


Hauryliuk V, Mitkevich VA, Draycheva A, Tankov S, Shyp V, Ermakov A, Kulikova AA, Makarov AA, Ehrenberg M.
Thermodynamics of GTP and GDP binding to bacterial initiation factor 2 suggests two types of structural transitions.
J Mol Biol. 2009 Dec 11; 394(4):621-6

Tenson T, Hauryliuk V.
Does the ribosome have initiation and elongation modes of translation?
Mol Microbiol. 2009 Jun; 72(6):1310-5


Atkinson GC, Baldauf SL, Hauryliuk V.
Evolution of nonstop, no-go and nonsense-mediated mRNA decay and their termination factor-derived components.
BMC Evol Biol. 2008 Oct 23; 8:290

Hauryliuk V, Mitkevich VA, Eliseeva NA, Petrushanko IYu, Ehrenberg M, Makarov AA.
The pretranslocation ribosome is targeted by GTP-bound EF-G in partially activated form.
Proc Natl Acad Sci U S A 2008 Oct 14; 105(41):15678-83.

Hauryliuk V, Hansson S, Ehrenberg M.
Cofactor dependent conformational switching of GTPases.
Biophys J. 2008 Aug; 95(4):1704-15.

Andreev D, Hauryliuk V, Terenin I, Dmitriev S, Ehrenberg M, Shatsky I.
The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome.
RNA 2008 Feb; 14(2):233-9.


Hauryliuk VV.
GTPases of prokaryotic translational apparatus
Mol Biol (Mosk). 2006 Sep-Oct; 40(5):769-83

Hauryliuk V, Zavialov A, Kisselev L, Ehrenberg M.
Class-1 release factor eRF1 promotes GTP binding by class-2 release factor eRF3.
Biochimie 2006 Jul; 88(7):747-57.

Hauryliuk V, Ehrenberg M.
Two-step selection of mRNAs in initiation of protein synthesis.
Mol. Cell. 2006 Apr 21; 22(2):155-6.


Zavialov AV, Hauryliuk VV, Ehrenberg M.
Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs.
J Biol. 2005; 4(2):9
discussed in Research News in The GTPase switch in ribosomal translocation, Pete Moore, J Biol. 2005; 4(2):7, and in Research Highlights, Nature 2005 Sep 8; 473, 172-173

Zavialov AV, Hauryliuk VV, Ehrenberg M.
Splitting of the posttermination ribosome into subunits by the concerted action of RRF and EF-G.
Mol. Cell. 2005 Jun 10; 18(6):675-86

Books and book chapters:

Atkinson GC and Hauryliuk V.
Evolution and function of RelA/SpoT Homologue (RSH) proteins.
in Encyclopedia of Life Sciences, John Wiley & Sons, Ltd; February 2012, DOI: 10.1002/9780470015902.a0023959

Hauryliuk V.
Translational GTPases. Structural, biochemical and thermodynamical aspects.
Saarbrücken VDM  Verlag; 2009.

Ehrenberg M, Hauryliuk V, Crist C, Nakamura Y.
Translation termination, prion [PSI+] and ribosomal recycling.
in Translational Control in Biology and Medicine, Edited by Mathews MB, Sonenberg N, Hershey JWB, 7th edn. New York: Cold Spring Harbour Press; 2006: 173-196.

Jokes aside:

Peil O, Hauryliuk V.
A new spin on spinning your samples: balancing rotors in a non-trivial manner
arXiv:1004.3671v1, 2010

Hauryliuk V.
Evolution of translational machinery: could translation termination come into being before elongation?
J Theor Biol. 2007 Oct 7;248(3):574-8

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