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Proteomics


Scientific coordinator: Gunnar Wingsle, This email address is being protected from spambots. You need JavaScript enabled to view it.

Contact: Thomas Kieselbach, This email address is being protected from spambots. You need JavaScript enabled to view it.

The proteomic platform at UPSC is a part of the Umeå Protein Analysis (UPA) Facility. It was initiated in 2003 and provides infrastructure for research in protein science and proteomics. The facility is located at the Chemistry and Biology Center (KBC) of Umeå University and at UPSC. The work of UPA facility has from the beginning had a broad perspective that included the entire range of research in life science at Umeå University, the University hospital, and SLU. As a result of a work that combines education, collaborations, and services relevant advances in the development of research using proteomics and mass spectrometry based protein science were achieved.


The proteomic platform provides infrastructure, service and development for research and education in protein science, proteomics and mass spectrometry. Its instrumental and bioinformatics equipment and infrastructure was enabled through considerable investments from Umeå University, SLU, and The Swedish Research Council. As a result a complete proteomics workflow is now available for the analysis of post-translational modifications (electron dissociation mass spectrometer), which will considerably improve the current opportunities for all research in protein science in Umeå.

 

The main current available services are:

  • 2-D DIGE automated spot picking
  • protein analysis using MALDI-TOF and ESI-Q-TOF mass spectrometry.

The bioinformatics infrastructure includes:

  • on-line booking of instruments
  • data transfer
  • a local Mascot server providing access to many important sequence databases.

 

Technical equipment:

  • MALDI-TOF mass spectrometry: Voyager DE-STR mass spectrometer from Applied Biosystems is used for both research and development, and protein analysis services
  • Electrospray mass spectrometry: Q-tof Ultima ESI mass spectrometer from Micromass that can be combined with a nanoACQUITY UPLC from Waters for LC/MS/MS applications. Services on this instrument include currently protein identification by LC/MS/MS analysis.

 

Teaching in proteomics methods:

The personnel of the proteomic platform is not only organizing own courses, but has also contributed to other courses including:

  • Applied Mass Spectrometry
  • Functional Genomics Course.

The future teaching activities will continue this work and also contribute to the KBC Research School for graduate students. For no experienced scientists, the platform offers training courses two to three times a year. The personnel has more than five years experience in mass spectrometry training. The training includes usually three parts: theoretical introduction to protein science, individual laboratory training, and a practical task to practice the newly learned knowledge. Researchers, who are properly trained, are welcomed to use the MALDI-TOF mass spectrometer for own research.


Contact:
This email address is being protected from spambots. You need JavaScript enabled to view it.
Group leader at the Umeå Plant Science Centre and platform manager

This email address is being protected from spambots. You need JavaScript enabled to view it., researcher
Department of Chemistry
application chemist for Umeå Protein Analysis Facility

Robert Nilsson,50% technical position
SLU, Service on proteomic and metabolomic platforms

Emmanuelle Charpentier:
MIMS is characterized by
ECharpentier about MIMS 2015 1

Emmanuelle Charpentier, Alumna at MIMS, now Director of the Max Planck Unit for the Science of Pathogens and Director of the Department of Regulation in Infection Biology, Max Planck Institute for Infection Biology, Berlin, Germany.

Link to the Emmanuelle Charpentier Lab

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