UMU logo

Postdoctoral Research Opportunity in Infection Biology at Umeå University, Sweden

Project: The type VI secretion system of Francisella tularensis 

PI: Anders Sjöstedt

The aim is to delineate the regulation of assembly and the functions of the components of the Type VI secretion systems (T6SS) of Francisella tularensis (Ft). Ft encodes an atypical T6SS, which allows this highly virulent, intracellular bacterium to invade and replicate within the cytosol of eukaryotic cells and the T6SS is thereby essential for Ft virulence. While T6SSs have been the focus of an intense research effort for over a decade, our understanding is still incomplete regarding many of the functions of T6SSs in general and that of Ft in particular.

The research will be carried out by generating specific mutants and constructs encoding tagged T6SS components. These will then be tested by a variety of techniques, including several microscopic techniques, to detect the localization and secretion of the proteins, as well as their protein interaction partners. One focus will be on the baseplate, the foundation on which the external part of the machinery rests. Detailed structural knowledge is very limited regarding the Ft baseplate, a consequence of the low sequence identity between Ft T6SS components and those of prototypic T6SSs. We are currently conducting detailed analysis by means of for example two-hybrid interaction analysis, immunoprecipitation, ascorbate peroxidase 2 (APEX)-tagging, and confocal and electron microscopy methods. 

Application deadline: 7 January 2021. Continue to read for all details and how to apply. 

We have recently identified a critical role of the ClpB chaperone (Alam et al., Scientific Reports, 2018) and made the novel finding that its role for the control of T6S is dissociated from its role as essential for the heat shock response (Alam et al., Plos Pathogens, 2020). To further understand the underlying mechanisms is one aim. Much Ft research has focused on the control of T6SS assembly in the environmental bacterium F. novicida. Therefore, another aim will be to study clinically important Ft subspecies to see if the mechanism is conserved, with a special focus on ClpB and its role for the assembly/disassembly of the T6SS.

Apply here:

Contact: Anders.Sjostedt(at)umu.se

Webpages for more information:

https://www.umu.se/en/research/groups/anders-sjostedt/

https://www.umu.se/personal/anders-sjostedt/

 

Emmanuelle Charpentier took the Nobel Prize in Chemistry

Emmanuelle has been awarded jointly with Jennifer Doudna the 2020 Nobel Prize in Chemistry for discovering the groundbreaking CRISPR-Cas9 gene editing technology. She is a former group leader at MIMS, honorary doctor at Umeå University and former visiting professor at UCMR.

Movie by Knut and Alice Wallenberg Foundation (https://kaw.wallenberg.org/)

MIMS is characterized by
ECharpentier about MIMS 2015 1

Emmanuelle Charpentier, Alumna at MIMS, Director of the Max Planck Unit for the Science of Pathogens, Berlin, Germany.

Link to the Emmanuelle Charpentier Lab

Battling antibiotic resistance

movie by Knut and Alice Wallenberg Foundation, with participation of scientists from MIMS and UCMR:
Or watch the original movie on:
https://kaw.wallenberg.org/

Research about infectious diseases:

Oliver Billker in movie of Knut and Alice Wallenberg Foundation:
Or watch the original movie on:
https://kaw.wallenberg.org/

Emmanuelle Charpentier
about her research at MIMS

footer all slides 2014-02-06


Copyright © 2019 by The Laboratory for Molecular Infection Medicine Sweden (MIMS). All rights reserved.